The donor pool for heart transplants is severely limited and there is still a legal problem of brain death. This study assessed the function of hearts "absolute anoxic" for ten minutes after asphyxia by perfusing the hearts on a Langendorff
apparatus
for 45 minutes with Krebs-Henseleit buffer at 37℃ at 80 cm H2O.
Forty isolated rat hearts were divided into four groups. Ten control hearts(group l)were perfused on the circuit without intervening ischemia. Ten hearts(group 2) were harvested, quickly flushed with 5cc of cold University of Wisconsin solution,
and
stored in the same cold solution for 4 hours. Ten hearts(group 3) were excised, excised, quickly flushed with 5 cc of cold Stanford cardioplegic solution and stored in cold saline solution for 4 hours. Ten asphyxiated hearts(group 4) had warm
ischemia
for ten minutes and were perfused with 5cc of cold Stanford cardioplegia containing 7,500 units of urokinase to dissolve intravascular clots, and stored in cold saline solution for 1.5 hours.
Time of spontaneous defibrillation(TSD) after perfusion was significantly longer in group 2, group3 and group 4 than in group 1. TSD in group 3 and group 4 was significantly longer in comparison to that of group 2. Left ventricular developed
pressure(LVDP) at 15 minutes was significantly lower in group 3 and group 4 than in group l and group 2. I group 4, LVDP at 30 minutes and 45 minutes was significantly lower compared with that in group 1.
In conclusion, asphyxiated rat hearts which had absolute anoxia for 10 minutes after asphyxia showed relatively satisfactory cardiac function.
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